In order to extract membrane proteins, cells need to be disrupted by either ultrasound or high-pressure methods. The most common approach is to homogenize tissues or cells into an emulsified mixture in a buffered aqueous solution. A major concern during this process is that the target membrane proteins will be exposed to the proteases released from subcellular compartments and undergo proteolysis-caused degradation The proteolysis of the target proteins can be prevented or reduced by the addition of protease inhibitors. AlpHelix has developed potent protease inhibitors to prevent target proteins from proteolysis.