Case 3:Resolving the Cryo-EM structures of GPCR-Inhibitor Complexes by Fusion Protein Strategy
| 2 weeks | 1 week | 1 day | 1-2 weeks | 1 week | 1-2 weeks | 1 week |
| Generation of expression constructs | Overexpression and purification of proteins in HEK293 cells | Formation of complexes with small molecule compounds and preparation of frozen samples | Preliminary collection and processing of cryo-EM data, screening and evaluation of sample quality | High-quality cryo-electron microscopy data collection | Reconstruction of high-resolution electron density from data processing | Build and refine protein structural models to obtain high-precision complex structures |
